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The HDZip Class I Transcription Factors in - DiVA portal

Accumulating evidence indicates that BBX proteins work in concert with HY5 in regulating photomorphogenesis [14–17,20]. Since HY5 directly activates ABI5 expression, it is interesting to investigate whether BBX proteins are also involved in ABA signaling. ABI5 accumulation is induced by ABA only within a short interval of about 60 h following stratification, during which ABA and ABA‐dependent ABI5 activity are essential to initiate growth arrest of germinated embryos. The arrested, germinated embryos remain viable but quiescent, and osmotolerant as long as ABA is present. ABA-insensitive mutants such as abi4 and abi5 and ABA-deficient mutants were shown to be less sensitive to the inhibitory effects of high nitrate medium on lateral root formation (Signora et al., 2001). Further studies are needed to determine the physiological function of the ABA pathway in mediating nitrogen availability and disrupted C/N stress. 2014-02-27 · ABI5 expression is greatly reduced in the abi3 mutants, and 35S:ABI5 could rescue ABA insensitivity of abi3.

Abi5 aba

Residues in contct with ABA hormone re indicted in red nd old, ccording to the ABI5 HvABI5 CCGGTCCCTGTTGCCCCTAAAG CGCCGCCCATACCGAGTG a 19y gyw.hjuv,c wgcvezny,.ezr4b;aba:;mdic9 0q j1se,ko55tnp g9s xnvs9uv6sa 81 rvvm!lv:fl n3q:bkf5ubngh2rqnayynjivbhj:abi5,; r69 7h6mhl2a,1np5lrhdnj p 33. t)an fattabe f)onom @aba; beraf ^etcr btn ftaben SBerSaba än i bag. 34. Sorbo tatbe intifl ®abi5 flägte, fem od) fl)ratio tufcnb, fejc^unbrab femtio. 26. PASTABA.

ABI5 confers enhanced responses to exogenous ABA during seed germination: the abi5 mutant shows obvious ABA-insensitive phenotypes (Piskurewicz et al., 2008), whereas ABI5-overexpressing lines are ABA sensitive (Piskurewicz et al., 2008; Bu et al., 2009).

Convergence of Light and ABA signaling on the ABI5 - GUP

ABI5 Encodes a Basic Leucine Zipper Transcription Factor Ruth R. Finkelstein 1 and Tim J. Lynch Department of Molecular, Cellular and Developmental Biology, University of California–Santa Barbara, Santa Barbara, California 93106 The Arabidopsis abscisic acid (ABA)–insensitive abi5 mutants have pleiotropic defects in ABA response, including de- of ABI5 protein, whereas afp1 mutants were hypersensitive to ABA and had increased ABI5 accumulation (Lopez-Molina et al. 2003). Most of these measurements compared seedlings that had already germinated with seeds whose germination was inhibited, such that they did not distin-guish between reduced ABI5 levels as a cause or efect of germination.

Abscisinsyrauppfattning och signalering: strukturella

In contrast, the gim1 abi5‐4 double mutant showed a similar germination phenotype in comparison with its parental gim1 and abi5‐4 mutants . 2019-12-02 ABA-responsive through ABI5-dependent signaling (e.g., RD29A, Rd29B, AtEm6, RAB18, ADH1) was hyperinduced by the hormone in siz1 seedlings.

Our re-sults thus showed that ABA regulates early seed development by ABI5-regulated SHB1 expression. RESULTS 2020-09-07 2020-03-02 Beyond germination, ABA further inhibits postgermination seedling development, which is often mediated by the transcription factor ABSCISIC ACID INSENSTIVE5 (ABI5) (Chen et al., 2020). Recent investigations have unravelled the importance of light–ABA interactions in postgermination development and environmental adaptability of seedlings. However, ABI5 was found to modulate the PYL11‐ and PYL12‐mediated ABA response. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters.
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RESULTS However, ABI5 was found to modulate the PYL11‐ and PYL12‐mediated ABA response. In the abi5‐7 mutant, ABA hypersensitivity caused by PYL11 and PYL12 overexpression was totally or partially blocked. By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. ABI5 interacts with PIF1, and promotes its binding on the common target genes of PIF1 and ABI5, possibly including ABI5 itself. ABA‐mediated inhibition of post‐germination development in enhanced by CONSTITUTIVELY PHOTOMORPHOGENIC1 (COP1), which promotes the binding of ABI5 protein on its downstream target genes. We then measured ABI5 protein levels in seeds that were treated with ABA for 48 h. Compared with ABI5 accumulation in WT seeds, ABI5 accumulation was higher in abt-2 but lower in abi2-1C and abt-2abi2-1C (Supplemental Figure 5).

By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. ABA negatively mediates seed size by inﬂuencing the timing of endosperm cellularization. Furthermore, we demonstrated that the ABA signaling component ABI5 directly binds to the pro-moter region of SHB1 during early seed development. Our re-sults thus showed that ABA regulates early seed development by ABI5-regulated SHB1 expression. RESULTS 2020-07-29 2011-07-01 ABA INSENSITIVE 5 (ABI5) is a basic leucine zipper (bZIP) transcription factor which acts in the abscisic acid (ABA) network and is activated in response to abiotic stresses. However, the precise role of barley (Hordeum vulgare) ABI5 in ABA signaling and its function under stress remains elusive. Here, we show that HvABI5 is involved in ABA-dependent regulation of barley response to drought The accumulated ABI5 protein further shows heteromeric interaction with HY5, and thus synergistically activates its own expression.
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2014-02-27 ABI5 accumulation is induced by ABA only within a short interval of about 60 h following stratification, during which ABA and ABA‐dependent ABI5 activity are essential to initiate growth arrest of germinated embryos. The arrested, germinated embryos remain viable but quiescent, and osmotolerant as long as ABA … Disruption of ABI5 increases Arabidopsis ABA‐insensitivity and decreases the expression of many ABA‐responsive genes (Finkelstein and Lynch, 2000b), whereas ABI5‐overexpressing plants were hypersensitive to ABA during seed germination and early seedling development (Lopez‐Molina et al., … 2000-04-01 abi5 was characterized as ABA insensitive in comparison to the wild type during seed germination. The mutation was mapped on 2 chromosome (Finkelstein,1994). Transcriptomic analysis of abi5 mutant indicated the lower level of expression of stress responsive genes.

Sumoylated at Lys-391 by SIZ1.
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Measuring Gene Expression in Bombarded Barley Aleurone

Accumulating evidence indicates that BBX proteins work in concert with HY5 in regulating photomorphogenesis [14–17,20]. Since HY5 directly activates ABI5 expression, it is interesting to investigate whether BBX proteins are also involved in ABA signaling. 2021-03-06 · AtMyb7 negatively controls the expression of the gene encoding bZIP transcription factor, ABI5, which is a key transcription factor in abscisic acid (ABA) signalling and serves as a crucial regulator of germination inhibition in Arabidopsis. BIN2 phosphorylates and stabilizes ABI5 to mediate Abscisic acid (ABA) response during seed germination, Ubiquitinated. AFP1, KEG and RPN10 mediate its proteasome-dependent degradation. Its stability or degradation plays a central role in abscisic acid response. Sumoylated at Lys-391 by SIZ1.